●問題1

貨號：A137

品名：Factor H

品牌：complementtech

原文：

Q:May I ask if different batches of this product (A137) can be mixed and if this antibody has been tested for endotoxins?

A:Sorry for the delay.

Yes, you can mix the lots.  You will have to test, etc for activity.  If endotoxin tests were performed it would be listed on the applicable CoA.  If it is not listed then we did not test for endotoxins.

譯文：

問：請問該產品（A137）的不同批次能否混合使用？該抗體是否做過內毒素檢測？

答：抱歉回復延遲。

不同批次的產品可以混合使用，你需要對混合后的產品進行活性等相關檢測。如果產品做過內毒素檢測，相關結果會在對應的分析報告中列出；若報告中未提及，則說明該產品未進行內毒素檢測。

●問題2

貨號：10-0027-1

品名：Protein L Ligand Leakage ELISA

品牌：Medicago

原文：

Q:Customer feedback: Why has the expiration date of protein L reference （lot：285004） from the same batch been extended from 2025-10 to 2026-8 and 2027-8? Have you retested the protein L reference? If so, could you provide relevant statements and data?

A:Yes, we have extended the expiration date for the entire kit, including the references. There is no better proof than these certificates that the references have the same expiration date as the kit.

譯文：

問：客戶反饋：同批次的蛋白L標準品（批號：285004）有效期為何從2025年10月分別延長至2026年8月和2027年8月？貴司是否對該蛋白L標準品重新進行了檢測？若有，煩請提供相關說明及檢測數據。

答：我們已延長了整套試劑盒（包括參考品在內）的有效期。這些證書就是參考品與試劑盒有效期一致的最有力證明。

●問題3

貨號：M050-1

品名：GPIbbeta (CD42c)Xia.C3 Rat IgG2a FITC; 300 tests

品牌：emfret

原文：

Q:The customer would like to inquire about the concentration of this antibody (M050-1)

A:Thank you for your inquiry.

We titrate the fluorescence-conjugated antibodies in relation to our negative controls (P190-X) so that optimal results are achieved. The ab concentration is not determined afterwards.

I am sorry to have no other information.

譯文：

問：客戶想咨詢該抗體（M050-1）的濃度是多少？

答：感謝你的咨詢。

我們以P190-X陰性對照為參照，對熒光標記抗體進行滴定，以確保實驗能得到最佳結果，后續并未測定該抗體的濃度。

很抱歉，目前沒有更多相關信息可以提供。

●問題4

貨號：A137

品名：Factor H

品牌：complementtech

原文：

Q:Hello, may I ask how the extinction coefficient of A137 was obtained? Was it measured experimentally or by other means? It differs quite significantly from the theoretical extinction coefficient based on the sequence. Thank you.

A:Hi Jane,

We have determined FH concentration spectrophotometrically using an extinction coefficient A280 nm (1% solution) of 12.4 (Ref#1). Dr. MK. Pangburn has extensively worked on Factor H. Based on the explanation given by Dr. MK Pangburn in his paper here is the summary: Extinction coefficients reported for factor H have been reported ranging from 9.0 to 19.5 (Ref#1 to 4). The calculated extinction coefficient from the sequence (Ref#4) that has been corrected for the large amount of polysaccharide in factor H is 13.2. Despite these differences, which are typical for a widely studied protein, the value of 12.4 is in the middle of the reported range and is closest to the calculated value after correction for the high content of polysaccharide.  

References

Pangburn, M. K., and H. J. Mu¨ller-Eberhard. 1983. Kinetic and thermodynamic analysis of the control of C3b by the complement regulatory proteins factors H and I. Biochemistry 22: 178–185).

Sim, R. B., and R. G. Discipio. 1982. Purification and structural studies on the complement system control protein IH (factor H). Biochem. J. 205: 285–293.

Nagaki, K., K. Iida, M. Okubo, and S. Inai. 1978. Reaction mechanims of 1H globulin. Int. Arch. Allergy Appl. Immunol. 57: 221–232.

Hakobyan, S., C. L. Harris, A. Tortajada, J. E. Goicochea de, A. Garcia-Layana, P. Fernandez-Robredo, C. S. Rodriguez de, and B. P. Morgan. 2008. Measurement of factor H variants in plasma using variant-specific monoclonal antibodies: application to assessing risk of age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 49: 1983–1990.

譯文：

問：您好，請問A137的消光系數是如何確定的？是通過實驗測定的，還是通過其他方法得出的？該數值與根據序列計算的理論消光系數存在較大差異，謝謝。

答：你好，簡：

我們采用分光光度法，以12.4的280nm摩爾消光系數（1%溶液，參考文獻1）測定了補體因子H（FH）的濃度。MK·潘伯恩博士對補體因子H有深入的研究，根據其論文中的解釋，現總結如下：已有文獻報道的補體因子H消光系數范圍為9.0至19.5（參考文獻1至4）；通過氨基酸序列計算，并針對補體因子H中大量的多糖成分進行校正后，得到的消光系數為13.2（參考文獻4）。對于一種被廣泛研究的蛋白質而言，出現上述消光系數的差異是正常現象，而12.4這一數值處于已報道的范圍中間，且與經多糖高含量校正后的計算值最為接近。

參考文獻

潘伯恩·M·K、米勒-埃伯哈德·H·J，1983年，補體調節蛋白H因子和I因子調控C3b的動力學與熱力學分析，《生物化學》，22卷：178-185頁

西姆·R·B、迪西皮奧·R·G，1982年，補體系統調節蛋白IH（H因子）的純化與結構研究，《生物化學雜志》，205卷：285-293頁

長脅·K、飯田·K、大久保·M、稻井·S，1978年，1H球蛋白的反應機制，《國際過敏反應與應用免疫學文獻》，57卷：221-232頁

哈科布揚·S、哈里斯·C·L、托爾塔哈達·A等，2008年，利用變異型特異性單克隆抗體檢測血漿中補體因子H變異體及其在年齡相關性黃斑變性風險評估中的應用，《眼科研究與視光科學》，49卷：1983-1990頁

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