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發布時間：2018/11/2
點擊次數：644
Capricorn Scientific成立于2013年，是一家新的德國細胞培養基，血清和試劑生產商。我們生產的主要重點是生產用于生物技術，診斷和研究應用的高質量動物血清。嚴格的測試程序以及全面的可追溯性記錄和文檔保證了我們產品的和可重復的質量。
作為一家規模較小的公司，我們可以靈活地提供各種傳統和定制產品，以更低的成本，更短的交付周期和個性化的客戶服務滿足當今市場不斷增長的需求。
我們的技術，經驗豐富的專家團隊多年來一直致力于細胞培養領域。我們致力于為活躍的細胞培養公司提供高質量，可靠性和服務。
Capricorn FM1-F說明書
冷凍一種含胎牛血清的細胞保藏培養基
貓編號：FM1-F(50毫升)
產品闡述，產品說明
凍干粉是一種含有10%DMSO和20%FBS的冷凍保護介質。所用的血清在細胞保存期間被廣泛測試以保護細胞。
申請
-冷凍保存多種高活力的細胞類型
-現成的解決方案
商品概述
不育試驗
貯存≤-15°C.凍干粉是一種光敏溶液.在運輸和儲存期間，應該保護它不受光線的影響。
凍結議定書
在冷凍保存前，應檢查細胞是否受到污染。FreezeMe可以與任何標準的冷凍協議一起使用。
懸浮培養物的低溫保存
-計算冷凍保存的活細胞數。細胞應處于生長中期。離心5 min至顆粒細胞(200~400 g)。把上清液移到小的 在不打擾細胞的情況下。
-預冷(4°C~8°C)的復蘇細胞(5x106~107細胞/ml)。
-進入低溫儲存瓶。將小瓶置于4°C，5 min內開始冷凍。細胞以1°C/min的速度緩慢冷凍(可編程冷卻器或將小瓶放入島葉) 在攝氏-70度至攝氏-90度的冷凍機內放置箱)。
-然后將儲存瓶轉移到液氮儲存。
貼壁培養的冷凍保存
-用溫和的解離劑將細胞與底物分離。特別是敏感細胞使用酸性磷酸酶(CAT)。否避免細胞損傷。必要時滅活解離劑。
-在完整的培養基中復蘇分離的細胞，并建立可行的細胞計數。
離心5 min至顆粒細胞(200~400 g)。將上清液移至小體積，而不干擾細胞。
-預冷(4°C~8°C)的復蘇細胞(5x106~107細胞/ml)。
-進入低溫儲存瓶。將小瓶置于4°C，5 min內開始冷凍。細胞以1°C/min的速度緩慢冷凍(可編程冷卻器或將小瓶放入島葉) 在攝氏-70度至攝氏-90度的冷凍機內放置箱)。
-然后將儲存瓶轉移到液氮儲存。
凍存細胞可通過以下程序解凍
離心法，離心過濾
-從儲存中取出細胞，在37°C水浴中迅速解凍。摩羯座科學推薦使用經批準的安全護目鏡來保護眼睛。我們還建議使用安全手套來保護 裸露的皮膚。-將1至2毫升的解凍細胞放入25毫升的*培養基中。輕輕混合細胞懸液。
-80 g離心2~3 min。
-檢查細胞顆粒的上清和可見度。在不干擾細胞的情況下丟棄上清液。
-輕輕地將細胞重新懸浮在完整的培養基中，并進行可行的細胞計數。
-打平細胞。細胞接種量應至少為3x105個活細胞/ml。
直接電鍍
-從儲存中取出細胞，在37°C水浴中迅速解凍。摩羯座科學推薦使用經批準的安全護目鏡來保護眼睛。我們還建議使用安全手套來保護 裸露的皮膚。
-板細胞直接與完整的生長培養基。每1毫升冷凍細胞使用10至20毫升完整培養基。細胞接種量應至少為3x105細胞/ml。
-培養細胞12至24小時，用新鮮的*培養基替代培養基，去除冷凍保存液。
我們建議解凍程序1，特別是當處理敏感細胞。
預防措施及免責聲明
本產品僅供研究用。請查閱材料安全數據表，以獲取有關危險和安全處理做法的信息。
警告：冷凍Me含有二甲基亞砜(DMSO)。不要吸入氣體/煙霧/蒸汽/噴霧。避免接觸眼睛和皮膚。對眼睛、呼吸系統和皮膚有刺激性。S-23 S-24/25。
我們生產和測試了我們的生物加工和細胞培養血清產品，用于不同的哺乳動物細胞培養應用。我們提供各種各樣的特色血清：
液體培養基為所有細胞培養應用提供基本營養。我們的每種高質量細胞培養基都是根據初公布的配方或對培養基的一致性能和穩定性*的修飾而制造的。使用摩羯座培養基是保證細胞成功生長的宜方法。
我們提供各種產品：
來自摩羯座的試劑和補充劑允許您定制細胞培養系統的生長條件。所有產品都經過嚴格測試和設計，以提供可重復性和良性能。
我們提供各種各樣的產品：
Capricorn Scientific產品目錄：
貨號 | 品名 | 規格 | 價格 |
DMEM-LPA | DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-LPSTA | DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate | 500 ml | 9.50 € |
DMEM-LPXA | DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-HA | DMEM High Glucose (4.5 g/l), with L-Glutamine | 500 ml | 6.40 € |
DMEM-HHSTA | DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES | 500 ml | 9.50 € |
DMEM-HPA | DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-HPSTA | DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate | 500 ml | 7.00 € |
DMEM-HPXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-HXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine | 500 ml | 6.40 € |
DMEM-HXRXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red | 500 ml | 10.50 € |
DMEM-12-A | DMEM/Ham’s F-12, with L-Glutamine | 500 ml | 8.60 € |
DMEM-12-HXA | DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES | 500 ml | 8.60 € |
HAM-12-A | Ham’s F-12, with L-Glutamine | 500 ml | 9.50 € |
IMDM-A | IMDM, with L-Glutamine, without Supplements | 500 ml | 9.00 € |
L15-XA | Leibovitz’s L-15 Medium, w/o L-Glutamine | 500 ml | 12.40 € |
MCC-A | McCoy’s Medium, with L-Glutamine | 500 ml | 12.70 € |
M199-A | Medium 199, with L-Glutamine | 500 ml | 8.90 € |
MEM-A | MEM with Earle’s Salts, with L-Glutamine | 500 ml | 7.20 € |
MEM-STA | MEM with Earle’s Salts, with Stable Glutamine | 500 ml | 9.40 € |
MEM-XA | MEM with Earle’s Salts, w/o L-Glutamine | 500 ml | 6.40 € |
MEMA-RXA | MEM Alpha Modification, w/o L-Glutamine, with Nucleosides | 500 ml | 19.90 € |
MEMA-XRXA | MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides | 500 ml | 13.00 € |
RPMI-A | RPMI 1640, with L-Glutamine | 500 ml | 6.40 € |
RPMI-HA | RPMI 1640, with L-Glutamine, with 25 mM HEPES | 500 ml | 9.90 € |
RPMI-HXA | RPMI 1640, w/o L-Glutamine, with 25 mM HEPES | 500 ml | 9.50 € |
RPMI-STA | RPMI 1640, with Stable Glutamine | 500 ml | 8.40 € |
RPMI-XA | RPMI 1640, w/o L-Glutamine | 500 ml | 5.80 € |
RPMI-XRXA | RPMI 1640, w/o L-Glutamine, w/o Phenol Red | 500 ml | 9.90 € |
FM1-F | FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum | 50 ml | 65.00 € |
FM2-F | FreezeMe Two, Cryopreservation Medium Serum-free | 50 ml | 70.00 € |
TP-A | TumorPlus 263, Complete Medium for Tumor Cells, with L-Glutamine | 500 ml | 29.90 € |
HP-A | HybridomaPrime, Hybridoma Medium, Serum-free | 500 ml | 34.90 € |
HY-A | HybridomaPlus, Hybridoma Medium, Protein-free | 500 ml | 39.90 € |
FP-A | FibroPlus 333 Complete Medium for Fibroblasts, with L-Glutamine | 500 ml | 29.90 € |
ERP-B | EndoPrime Complete Medium for C*tion of Primary Cells | 100 ml | on request |
ERP-A | EndoPrime Complete Medium for C*tion of Primary Cells | 500 ml | on request |
HPR-B | HepaPrime Complete Medium for C*tion of Primary Hepatocytes | 100 ml | on request |
HPR-A | HepaPrime Complete Medium for C*tion of Primary Hepatocytes | 500 ml | on request |
NKR-G | NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells | 20 ml | on request |
NKR-B | NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells | 100 ml | on request |
CHOA-500ML | CHO-ONE A | 500 ml | 45.00 € |
CHOE-500ML | CHO-ONE E | 500 ml | 49.00 € |
CHOE-1000ML | CHO-ONE E | 1000ml | 89.00 € |
CHOF1-100ML | CHO-ONE F1 | 100 ml | 23.00 € |
CHOF1-500ML | CHO-ONE F1 | 500 ml | 95.00 € |
CHOF2-10ML | CHO-ONE F2 | 10 ml | 15.00 € |
CHOF2-50ML | CHO-ONE F2 | 50 ml | 49.00 € |
CHO-K1 | CHO-ONE Starter Kit |
| 169.00 € |
CHO-K2 | CHO-ONE Expansion Kit |
| 950.00 € |
DMEM-LPA-P10 | DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 10 L | 14.90 € |
DMEM-LPA-P50 | DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 50 L | 62.90 € |
DMEM-HA-P10 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 15.50 € |
DMEM-HA-P50 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 66.50 € |
DMEM-HPA-P10 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 10 L | 15.50 € |
DMEM-HPA-P50 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 50 L | 66.50 € |
MEM-A-P10 | MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 14.90 € |
MEM-A-P50 | MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 62.90 € |
RPMI-A-P10 | RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 15.50 € |
RPMI-A-P50 | RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 66.50 € |
RPMI-HA-P10 | RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate | 10 L | 20.10 € |
RPMI-HA-P50 | RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate | 50 L | 89.90 € |
FBS-52B | Fetal Bovine Serum, Collected in Australia | 100 ml | on request |
FBS-52A | Fetal Bovine Serum, Collected in Australia | 500 ml | on request |
FBS-12B | Fetal Bovine Serum, Collected in South America | 100 ml | on request |
FBS-12A | Fetal Bovine Serum, Collected in South America | 500 ml | on request |
FBS-22B | Fetal Bovine Serum, Collected in USA | 100 ml | on request |
FBS-22A | Fetal Bovine Serum, Collected in USA | 500 ml | on request |
FBS-HI-12B | Fetal Bovine Serum, Heat Inactivated, Collected in South America | 100 ml | on request |
FBS-HI-12A | Fetal Bovine Serum, Heat Inactivated, Collected in South America | 500 ml | on request |
FBS-HI-22B | Fetal Bovine Serum, Heat Inactivated, Collected in USA | 100 ml | on request |
FBS-HI-22A | Fetal Bovine Serum, Heat Inactivated, Collected in USA | 500 ml | on request |
FBS-11B | Fetal Bovine Serum Advanced, Collected in South America | 100 ml | on request |
FBS-11A | Fetal Bovine Serum Advanced, Collected in South America | 500 ml | on request |
FBS-HI-11B | Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America | 100 ml | on request |
FBS-HI-11A | Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America | 500 ml | on request |
FBS-TET-12B | Fetal Bovine Serum, Tetracycline Negative, Collected in South America | 100 ml | on request |
FBS-TET-12A | Fetal Bovine Serum, Tetracycline Negative, Collected in South America | 500 ml | on request |
FBS-ES-22B | Fetal Bovine Serum, ES cell pre-tested, Collected in USA | 100 ml | on request |
FBS-ES-22A | Fetal Bovine Serum, ES cell pre-tested, Collected in USA | 500 ml | on request |
FBS-DIA-12B | Fetal Bovine Serum, Dialysed, Collected in South America | 100 ml | on request |
FBS-DIA-12A | Fetal Bovine Serum, Dialysed, Collected in South America | 500 ml | on request |
FBS-IG-12B | Fetal Bovine Serum, IgG Stripped, Collected in South America | 100 ml | on request |
FBS-IG-12B | Fetal Bovine Serum, IgG Stripped, Collected in South America | 500 ml | on request |
FBS-ED-12F | Fetal Bovine Serum Exosome Depleted | 50 ml | on request |
FBS-ED-12B | Fetal Bovine Serum Exosome Depleted | 100 ml | on request |
FBS-ED-12A | Fetal Bovine Serum Exosome Depleted | 500 ml | on request |
NCS-1B | Newborn Calf Serum | 100 ml | 12.00 € |
NCS-1A | Newborn Calf Serum | 500 ml | 38.00 € |
NCS-HI-1B | Newborn Calf Serum, Heat Inactivated | 100 ml | 13.10 € |
NCS-HI-1A | Newborn Calf Serum, Heat Inactivated | 500 ml | 43.00 € |
CS-1B | Calf Serum | 100 ml | 8.00 € |
CS-1A | Calf Serum | 500 ml | 29.50 € |
ABS-1B | Adult Bovine Serum | 100 ml | 8.00 € |
ABS-1A | Adult Bovine Serum | 500 ml | 29.50 € |
ABS-1C | Adult Bovine Serum | 1000 ml | on reques |
HOS-1B | Horse Serum | 100 ml | 7.20 € |
HOS-1A | Horse Serum | 500 ml | 32.00 € |
DHS-1B | Donor Horse Serum | 100 ml | 9.00 € |
DHS-1A | Donor Horse Serum | 500 ml | 35.00 € |
GOA-1B | Goat Serum | 100 ml | 24.00 € |
GOA-1A | Goat Serum | 500 ml | 105.00 € |
POR-1B | Porcine Serum | 100 ml | 10.50 € |
POR-1A | Porcine Serum | 500 ml | 39.00 € |
MOU-1B | Mouse Serum | 100 ml | 499.00 € |
RAT-1B | Rat Serum | 100 ml | 499.00 € |
RAB-1B | Rabbit Serum | 100 ml | 99.00 € |
HUM-3B | Human Serum, Type AB, Male | 100 ml | 134.00 € |
BSA-1U | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 50 g | 56.00 € |
BSA-1S | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 100 g | 77.00 € |
BSA-1V | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 250 g | on request |
BSA-1T | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 500 g | 299.00 € |
BSA-FAF-1U | Bovine Serum Albumin, Fatty Acid Free, Origin USA | 50 g | 99.00 € |
BSA-FAF-1S | Bovine Serum Albumin, Fatty Acid Free, Origin USA | 100 g | 178.00 € |
BSA-PF-1U | Bovine Serum Albumin, Protease Free, Origin USA | 50 g | 88.00 € |
BSA-PF-1S | Bovine Serum Albumin, Protease Free, Origin USA | 100 g | 151.00 € |
HSA-1R | Human Serum Albumin | 10 g | 56.00 € |
HSA-1U | Human Serum Albumin | 50 g | 227.00 € |
HSA-1S | Human Serum Albumin | 100 g | 420.00 € |
N2-K | N2 Supplement (100x), Serum-free | 5 ml | 45.00 € |
C21-H | NCS21 Supplement (50x), Serum-free | 10 ml | 45.00 € |
ITS-H | ITS (100x), Insulin, Transferrin, Selenium | 10 ml | 25.00 € |
HCS-F | Hybridoma Supplément, Serum-free | 50 ml | 99.00 € |
GLN-B | L-Glutamine, 200 mM | 100 ml | 8.00 € |
STA-B | Stable Glutamine, 200 mM | 100 ml | 14.50 € |
NPY-B | Sodium Pyruvate, 100 mM | 100 ml | 10.50 € |
NEAA-B | MEM Nonessential Amino Acids (100x) | 100 ml | 8.50 € |
ACC-1B | Accutase Cell Detachment Solution | 100 ml | 26.00 € |
ACM-1G | AccuMax Cell Detachment Solution | 20 ml | 29.00 € |
ACM-1F | AccuMax Cell Detachment Solution | 50 ml | 69.00 € |
TRY-1B | Trypsin-EDTA (0.05 %) in DPBS (1x) | 100 ml | 7.00 € |
TRY-1B10 | Trypsin-EDTA (0.5 %) in DPBS (10x) | 100 ml | 11.50 € |
TRY-2B | Trypsin (0.25 %) in DPBS (1x) | 100 ml | 7.00 € |
TRY-2B10 | Trypsin (2.5 %) in DPBS (10x) | 100 ml | 11.50 € |
TRY-3B | Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red | 100 ml | 9.50 € |
TRY-4B | Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red | 100 ml | 8.50 € |
AMP-B | Amphotericin B Solution (250 µg/ml) | 100 ml | 14.00 € |
AAS-B | Antibiotic/Antimycotic Solution (100x) | 100 ml | 14.00 € |
GEN-10B | Gentamicin Solution (10 mg/ml) | 100 ml | 50.00 € |
PS-B | Penicillin/Streptomycin (100x) | 100 ml | 8.00 € |
PSG-B | Penicillin/Streptomycin (100x), with L-Glutamine | 100 ml | 15.00 € |
MYX-B | MycoXpert, Mycoplasma Removal Reagent (50x) | 100 ml | 99.00 € |
LSM-B | Lymphocytes Separation Media, Density 1.077 g/ml | 100 ml | 12.00 € |
LSM-A | Lymphocytes Separation Media, Density 1.077 g/ml | 500 ml | 36.50 € |
FM1-F | FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum | 50 ml | 65.00 € |
FM2-F | FreezeMe Two, Cryopreservation Medium Serum-free | 50 ml | 70.00 € |
MYX-B | MycoXpert, Mycoplasma Removal Reagent (50x) | 100 ml | 99.00 € |
PBS-10XA | Dulbecco's PBS (10x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 13.00 € |
PBS-1A | Dulbecco's PBS (1x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 6.50 € |
PBS-2A | Dulbecco's PBS (1x), with Ca & Mg, w/o Phenol Red | 500 ml | 8.00 € |
HBSS-1A | Hanks' Balanced Salts (1x), with Ca & Mg, w/o Phenol Red | 500 ml | 7.50 € |
HBSS-2A | Hanks' Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 7.00 € |
HBSS-3A | Hanks' Balanced Salts (1x), w/o Ca & Mg, with Phenol Red | 500 ml | 7.50 € |
HEP-B | HEPES Buffer Solution (1 M) | 100 ml | 39.00 € |
PBS-1A-P10 | Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red | 10 L | 10.90 € |
PBS-1A-P50 | Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red | 50 L | 43.10 € |
Capricorn Scientific中國代理, Capricorn Scientific上海代理， Capricorn Scientific北京代理，Capricorn Scientific廣東代理， Capricorn Scientific江蘇代理Capricorn Scientific湖北代理,Capricorn Scientific天津代理, Capricorn Scientific黑龍江代理, Capricorn Scientific內蒙古代理, Capricorn Scientific吉林代理, Capricorn Scientific福建代理, Capricorn Scientific江蘇代理, Capricorn Scientific浙江代理, Capricorn Scientific四川代理,
上海起發實驗試劑有限公司是實驗試劑一站式采購服務商
1：強大的進口輻射能力，血清、抗體、耗材、大部分限制進口品等。
2：產品種類齊全，經營超過700多個品牌，基本涵蓋所有生物實驗試劑耗材。
3：提供加急服務，貨品一般1-2周到貨。
4：富有競爭力的價格優勢，絕大部分價格有優勢。
5：多年積累良好的信譽，大部分客戶提供貨到付款服務。客戶包括清華、北大、交大、復旦、中山等100多所高校，ROCHE，阿斯利康、國藥、fisher等藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批發，歡迎合作。
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