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Dianova DIA-100說明書

發(fā)布時間：2018/12/21

點(diǎn)擊次數(shù)：619

Dianova公司為德國的病理免疫組化產(chǎn)品供應(yīng)商，具備先進(jìn)的抗體制備工藝及嚴(yán)格的質(zhì)控系統(tǒng)，產(chǎn)品在上享有良好的聲譽(yù)。

Dianova DIA-100說明書

DIA-100-來自小鼠（AS02）的抗成纖維細(xì)胞（CD90，Thy-1）（Hu） - 不對稱。 - 1毫升

Art.-Nr.

DIA-100

特異性

成纖維細(xì)胞（CD90，Thy-1）

物種反應(yīng)性

人的

寄主物種

老鼠

同型

的IgG1

克隆

AS02

克隆性（單克隆/多克隆）

單克隆

應(yīng)用

免疫組化（冷凍切片）
免疫熒光
流式細(xì)胞儀
Western Blot
免疫沉淀免疫
組織化學(xué)（IHC）

共軛

非結(jié)合

格式

蛋白A / G純化（來自培養(yǎng)上清液）
凍干

產(chǎn)品系列/主題

CD標(biāo)記
神經(jīng)生物學(xué)

有可能的使用

僅供研究使用

溫度 - 儲存

2-8℃

搜索代碼

DIA100

制造商/品牌

dianova

流式細(xì)胞術(shù) - 抗CD90 / Thy1抗體（DIA-100，DIA-120）。抗體與人皮膚成纖維細(xì)胞強(qiáng)烈反應(yīng)，而它不染色淋巴細(xì)胞，角質(zhì)形成細(xì)胞，單核細(xì)胞，粒細(xì)胞，血小板，HUVEC和HDMC。單克隆抗體AS02（???）與合適的細(xì)胞標(biāo)記物（---）比較。

Western blot - 抗CD90 / Thy1抗體（DIA-100）。通過用0.6μg/ ml抗CD90 / Thy1抗體克隆AS02（DIA-100）進(jìn)行Western印跡檢測人成纖維細(xì)胞的細(xì)胞提取物中的CD90（Thy-1）蛋白。抗體AS02的CD90 / Thy-1檢測在還原和非還原條件下顯示30-32kDa的條帶，使用未還原的樣品具有更好的反應(yīng)性。由AS02結(jié)合的表位存在于15-kDa多肽鏈內(nèi)，而不存在于N-連接的寡糖側(cè)鏈或GPI錨結(jié)構(gòu)中。

免疫組化 - 抗CD90 / Thy1抗體（DIA-100）。在使用與磁珠偶聯(lián)的抗CD90抗體克隆AS02（DIA-100）消除污染的成纖維細(xì)胞之前（a，b）和（c）之前的甲狀腺細(xì)胞細(xì)胞培養(yǎng)物。用AS02免疫染色成纖維細(xì)胞（紅色），細(xì)胞核用haemalaun復(fù)染。
a）次傳代后的甲狀腺細(xì)胞培養(yǎng); 單個成纖維細(xì)胞（紅色）可見
b）第四次傳代后的甲狀腺細(xì)胞培養(yǎng); 可見許多成纖維細(xì)胞（紅色）
c）使用AS02磁珠消除成纖維細(xì)胞后的甲狀腺細(xì)胞培養(yǎng)物（第五代）。

免疫熒光/免疫細(xì)胞化學(xué) - 抗CD90 / Thy1抗體（DIA-100，DIA-120）。用抗CD90 / Thy1抗體克隆AS02（綠色）和抗脯氨酰羥化酶抗體（黃色）對培養(yǎng)的人皮膚成纖維細(xì)胞進(jìn)行免疫熒光雙重染色。（放大倍數(shù)x 300）

Dianova2019年產(chǎn)品價格表

貨號	品名	規(guī)格	價格	品牌
DIA-100	Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02)	200µg	4680	dianova
DIA-100-M	Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02)	20µg	0	dianova
DIA-120	Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) -FITC	100test	5360	dianova
DIA-530-P05	Anti-p53 (Hu) from Mouse (Clone: CC53)	0.5ml	4780	dianova
DIA-530-M	Anti-p53 (Hu) from Mouse (Clone: CC53)	0.1ml	0	dianova
DIA-670-P05	Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD	0.5ml	4780	dianova
DIA-670-P1	Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD	1ml	6500	dianova
DIA-670-M	Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD	0.1ml	0	dianova
DIA-700-P05	Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD	0.5ml	4780	dianova
DIA-700-M	Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD	0.5ml	0	dianova
DIA-AX1	Anti-ATRX (Hu) from Mouse (Clone: AX1)	0.5ml	6760	dianova
DIA-CAL-100	Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2)	0.1ml	5960	dianova
DIA-CAL-250	Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2)	0.25ml	13960	dianova
DIA-H09	Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD	0.5ml	9640	dianova
DIA-H09-M	Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD	0.1ml	2840	dianova
DIA-H09-L	Anti-IDH1 R132H (Hu) from Mouse (Clone: H09), prediluted, ready-to-use	8ml	9640	dianova
DIA-W09	Anti-IDH1 (Hu) from Rat (Clone: W09)	0.5ml	4960	dianova
DIA-TG1	Anti-TIGIT (Hu) from Mouse (Clone TG1)	0.5ml	20400	dianova
DIA-TG1-M	Anti-TIGIT (Hu) from Mouse (Clone TG1)	0.1ml	5720	dianova
DIA-TC8	Anti-CD8 (Hu) from Mouse (Clone TC8 )	0.5ml	9200	dianova
DIA-P16-OD	Anti-P16 (Hu) from Mouse (Clone JAP16)	0.1ml	7360	dianova
DIA-303	Anti-CD3 (Ms, FFPE) from Rat (Clone: HH3E)	0.5ml	6900	dianova
DIA-310	Anti-CD31 (Ms, FFPE) from Rat (Clone: SZ31)	0.5ml	8040	dianova
DIA-404	Anti-CD4 (Ms, FFPE) from Rat (Clone GHH8)	0.2ml	6900	dianova
DIA-808	Anti-CD8a (Ms, FFPE) from Rat (Clone GHH8)	0.2ml	6900	dianova
DIA-60	Anti-FasL, soluble (Hu) from Mouse (Clone: 94)	1ml	4760	dianova
DIA-900-100	Anti-HIS-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone: 13/45/31)	100µg	2960	dianova
DIA-900-200	Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone: 13/45/31)	200µg	5160	dianova
DIA-900-FITC	Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) -FITC	1ml	5960	dianova
DIA-900-BIOT	Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) -Biotin	100µg	3960	dianova
DIA-910-200-ABF	Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) Acid/BSA-free	200µg	4960	dianova
DIA.910-1MG-ABF	Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) Acid/BSA-free	1mg	19840	dianova
SCR-028841	IS Adhesion Slides	50pcs.	1620	dianova
SCR-028855	IS Adhesion Slides	5pcs.	詢價	dianova
SCR-028842	IS Adhesion Slides	100pcs.	2440	dianova
SCR-038447	Immunoselect Antifading Mounting Medium	15ml	960	dianova
SCR-038587	Immunoselect Antifading Mounting Medium	1ml	詢價	dianova
SCR-038448	Immunoselect Antifading Mounting Medium DAPI	15ml	1180	dianova
SCR-038588	Immunoselect Antifading Mounting Medium DAPI	1ml	詢價	dianova
SCR-038449	Immunoselect Antifading Mounting Medium PI	15ml	1180	dianova
SCR-072967	Immunoselect Mounting Medium without hardener	15ml	960	dianova
SCR-093035	Immunoselect Antifading Mounting Medium DAPI without hardener	15ml	1180	dianova
SCR-093036	Immunoselect Antifading Mounting Medium PI without hardener	15ml	1180	dianova
DIA-0302-I	p53-auto antibody ELISA-Kit (Human) CE-IVD	96test	10960	dianova

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